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Micropropagation of Neolamarckia macrophylla (Roxb.) Bosser to support ex situ conservation and breeding: multiplication, rooting, and acclimatization

Siti Halimah Larekeng (1,2)*, Nur Aida (3), Arif Nirsatmanto (4), Teguh Setyaji (5),
Muhammad Restu (1,6), and Djoko Iriantono (2)

1. Faculty of Forestry, Universitas Hasanudin, Jl. Perintis Kemerdekaan KM. 10, Makassar 90245,
South Sulawesi, Indonesia. *E-mail: sitih5h.82@gmail.com
2. Natural Heritage and Biodiversity Research Center, Universitas Hasanuddin, Jl. Perintis
Kemerdekaan KM. 10, Makassar 90245, South Sulawesi, Indonesia.
3. Regional of Seed/Seedling Forest Tree, South Sulawesi, Indonesia.
4. National Research and Innovation Agency, Gedung B.J. Habibie, Jl. M.H. Thamrin No. 8,
Jakarta Pusat 10340, Indonesia.
5. Local Government of Magelang Regency, Jalan Soekarno Hatta No. 59 Magelang, Central
Java, Indonesia.
6. Faculty of Vocational, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM. 10, Makassar
90245, South Sulawesi, Indonesia.
Abstract:

This research aimed at obtaining the appropriate media for in vitro multiplication, rooting, and acclimatization, as well as seedling quality, for the development of red jabon through tissue culture technique. Two experimental designs were used in this study: a factorial design for multiplication and rooting stages and a completely randomised design for acclimatization stage and seedling quality. The results showed that Murashige and Skoog (MS) medium, Woody Plant Medium (WPM), and Driver Kuniyuki Walnut (DKW) medium responded well during the multiplication stage. Neolamarckia macrophylla plantlets from WPM had the highest percentage of survival rate. The appropriate acclimatization medium for N. macrophylla is compost and sand (4:3). The sturdiness of stem and seedling quality index of the two-month-old seedling has already fulfilled the requirement for replanting to the field. High multiplication frequency ensures the efficiency of this protocol. This tissue culture method can be applied for the large-scale production and sustainable conservation of N. macrophylla, a vulnerable species for agroforestry of coastal regions for windbreak and soil reclamations.

Received: 20 February 2022 / Accepted: 29 June 2023 / Available online: 19 July 2023

Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
(Forestry Ideas, 2023, Vol. 29, No. 1) [Download]
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